KMID : 0358920230500030347
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Journal of the Korean Academy of Pedodontics 2023 Volume.50 No. 3 p.347 ~ p.359
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Effect of Polydeoxyribonucleotide on Human Periodontal Ligament Cells as a Storage Medium for Avulsed Tooth
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Ro Sang-Tae
Chae Yong-Kwon Lee Ko-Eun Kim Mi-Sun Nam Ok-Hyung Lee Hyo-Seol Choi Sung-Chul
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Abstract
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Objective: This study aimed to evaluate the suitability of polydeoxyribonucleotides (PDRN) as a storage medium for avulsed teeth.
Materials and Methods: The viability of human periodontal ligament (PDL) cells stored in Hank¡¯s balanced salt solution and PDRN solutions (concentrations, 10, 25, 50, and 100 ¥ìg/mL) and tap water was measured using the Cell Counting Kit-8 and Live/Dead assays. In addition, Nitric oxide detection and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the anti-inflammatory effect of PDRN.
Results: The viability of PDL cells stored in a 100 ¥ìg/mL PDRN solution was significantly higher than that of cells stored in the other solutions (p < 0.01). Furthermore, cells stored in 100 ¥ìg/mL PDRN solution demonstrated a significantly reduced NO production (p < 0.0001), and cells stored in 50 and 100 ¥ìg/mL PDRN solutions expressed significantly lower levels of tumor necrosis factor ¥á, interleukin (IL) -4, IL-6, and IL-10 (p < 0.01) compared to cells stored in HBSS.
Conclusion: The PDRN solution exhibited cell-preserving and anti-inflammatory effects on the PDL cells. The findings of this study can serve as a basis for further experiments directed at the development of an effective storage medium for avulsed teeth.
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KEYWORD
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Tooth avulsion, Storage media, Polydeoxyribonucleotide
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